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- 抗原
- AnsB
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-4371-0100
- Supplier
- Rockland
- 原理
- L-Asparaginase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.
- 产品特性
- Synonyms: rabbit anti-L-Asparaginase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
- 纯化方法
- Anti-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-AnsB antibody (Biotin)
适用: E. coli ELISA, WB, DB 宿主: 兔 Polyclonal Biotin
anti-AnsB antibody适用: E. coli ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Asparaginase Peroxidase conjugated antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and by ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- AnsB
- 背景
- Background: Anti-Asparaginase antibody detects Asparaginase. Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. Asparaginases are naturally occurring enzymes expressed and produced by microorganisms. Anti-Asparaginase Antibody is ideal for investigators involved in Immunology, Cell Signaling, Neuroscience and Signal Transduction research.
- 基因ID
- 1039460
- UniProt
- P00805
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