Protein G 抗体 (HRP)
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- 抗原 See all Protein G 抗体
- Protein G
- 适用
- Streptococcus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Protein G antibody is conjugated to HRP
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应用范围
- ELISA
- 交叉反应 （详细）
- Cross reactivity against Protein G from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].
- 免疫原
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Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Protein G Primary Antibody
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anti-Protein G antibody (FITC)
适用: Streptococcus FACS, IM 宿主: 兔 Polyclonal FITC
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- 应用备注
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Application Note: Anti-Protein G Peroxidase Conjugated Antibody has been assayed against 1.0 μg of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000-1:100,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Protein G
- Abstract
- Protein G 产品
- 背景
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Synonyms: HRP, Protein G Peroxidase, Anti-Protein G HRP Antibody
Background: Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein G Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.
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