Protein G 抗体 (Biotin)
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- 抗原 See all Protein G 抗体
- Protein G
- 适用
- Streptococcus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Protein G antibody is conjugated to Biotin
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应用范围
- ELISA, Dot Blot (DB)
- Supplier Product No.
- 200-406-138-0100
- Supplier
- Rockland
- 原理
- Protein G Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].
- 产品特性
- Synonyms: rabbit anti-Protein G Antibody biotin conjugation, Rabbit anti Protein G biotin, Rabbit anti PG biotin
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Protein G [Streptococcus species]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Protein G Primary Antibody
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anti-Protein G antibody (HRP)
适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal HRP
anti-Protein G antibody (FITC)适用: Streptococcus FACS, IM 宿主: 兔 Polyclonal FITC
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- 应用备注
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Application Note: Anti-Protein G Biotin Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:90,000 to 1:350,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:90,000 - 1:350,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Protein G
- Abstract
- Protein G 产品
- 背景
- Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein G antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
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