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- 抗原 See all Protein A 抗体
- Protein A
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适用
- Staphylococcus aureus
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Protein A antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Protein A from other sources is unknown.
- 纯化方法
- Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].
- 免疫原
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Immunogen: Protein A [Staphylococcus aureus]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Protein A Primary Antibody
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anti-Protein A antibody
适用: Staphylococcus aureus IP, Con 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, FM 宿主: 山羊 Polyclonal Texas Red (TR)
anti-Protein A antibody (HRP)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal HRP
anti-Protein A antibody (FITC)适用: Staphylococcus aureus FACS 宿主: 山羊 Polyclonal FITC
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, IM 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Immunohistochemistry Dilution: 1:500-1:2,500
Application Note: Anti-Protein A Biotin Conjugated has been assayed against 1.0 μg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:2,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Protein A
- Abstract
- Protein A 产品
- 别名
- alpha protein antibody, repB antibody
- 背景
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Synonyms: BAC, Protein A Biotin, Anti-Protein A BAC Antibody
Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein A antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
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