R-Phycoerythrin 抗体 (Rhodamine)
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- 抗原 See all R-Phycoerythrin products
- R-Phycoerythrin (Phycoerythrin, R-)
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This R-Phycoerythrin antibody is conjugated to Rhodamine
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 交叉反应 （详细）
- This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 20
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anti-Phycoerythrin, R- antibody (Texas Red (TR))
IA 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot.
IF Microscopy Dilution: 1:500-1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- R-Phycoerythrin (Phycoerythrin, R-)
- 别名
- R-Phycoerythrin (R-Phycoerythrin 产品)
- 物质类
- Chemical
- 背景
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Synonyms: TRITC
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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