(1 reference)-
- 抗原 See all R-Phycoerythrin products
- R-Phycoerythrin (Phycoerythrin, R-)
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This R-Phycoerythrin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 交叉反应 （详细）
- This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Phycoerythrin, R- antibody (Texas Red (TR))
IA 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Suitable for ELISA, western blotting, fluorescent assays, immunoprecipitation, immunodiffusion, conjugation to magnetic particles (beads) and most other immunological methods requiring lot-to-lot consistency, high titer and specificity. Although not specifically tested, this reagent should also be useful for FACS and other fluorescent immunoassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities." in: Current protocols in mouse biology, Vol. 5, Issue 3, pp. 247-270, (2016) (PubMed).
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: "Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities." in: Current protocols in mouse biology, Vol. 5, Issue 3, pp. 247-270, (2016) (PubMed).
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- 抗原
- R-Phycoerythrin (Phycoerythrin, R-)
- 别名
- R-Phycoerythrin (R-Phycoerythrin 产品)
- 物质类
- Chemical
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