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Pepsin A 抗体 (HRP)

Rockland 200-1376-0100 适用: Pig IHC, WB, ELISA, DB 宿主: 山羊 Polyclonal HRP
Rockland
产品编号 ABIN5596959
Supplier Product No.: 200-1376-0100
发货至: 中国
  • 抗原 See all Pepsin A 抗体
    Pepsin A
    适用
    • 3
    • 2
    • 1
    Pig
    宿主
    • 3
    • 2
    山羊
    克隆类型
    • 5
    多克隆
    标记
    • 4
    • 1
    This Pepsin A antibody is conjugated to HRP
    应用范围
    Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Dot Blot (DB)
    Supplier Product No.
    200-1376-0100
    Supplier
    Rockland
    原理
    Pepsin Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
    产品特性
    Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
    纯化方法
    Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Pepsin [Porcine Stomach]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product Pepsin A Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:125 - 1:625

    Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.

    Western Blot Dilution: 1:200 - 1:2,500

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Pepsin A
    别名
    PGA (Pepsin A 产品)
    背景
    Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.
    基因ID
    396892
    UniProt
    P00791
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