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Luciferase 抗体 (HRP)

适用: Firefly WB, ELISA 宿主: 山羊 Polyclonal HRP
产品编号 ABIN5596940
发货至: 中国
  • 抗原 See all Luciferase 抗体
    Luciferase
    适用
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    Firefly
    宿主
    • 56
    • 14
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    • 1
    山羊
    克隆类型
    • 50
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    多克隆
    标记
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    This Luciferase antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), ELISA
    交叉反应 (详细)
    No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].
    免疫原

    Immunogen: Luciferase [Photinus pyralis (Firefly)]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product Luciferase Primary Antibody
  • 应用备注

    Application Note: This product has been assayed against 1.0 μg of Luciferase [Photinus pyralis (Firefly)] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:2,000 - 1:30,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Windahl, Lagerquist, Andersson, Jochems, Kallkopf, Håkansson, Inzunza, Gustafsson, van der Saag, Carlsten, Pettersson, Ohlsson: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).

  • 抗原
    Luciferase
    Abstract
    Luciferase 产品
    别名
    hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody
    背景

    Synonyms: HRP, Luciferase Peroxidase, Anti-Luciferase HRP Antibody

    Background: Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract.

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