(1 reference)-
- 抗原 See all Luciferase 抗体
- Luciferase
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适用
- Firefly
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Luciferase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
- 纯化方法
- Anti-Luciferase Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].
- 免疫原
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Immunogen: Luciferase [Photinus pyralis (Firefly)]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Luciferase Primary Antibody
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anti-Luciferase antibody
适用: Firefly WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 6B8 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Firefly ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
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- 应用备注
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Application Note: Anti-Luciferase has been assayed against 1.0 μg of Luciferase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:80,000 to 1:310,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:25,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).
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: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).
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- 抗原
- Luciferase
- Abstract
- Luciferase 产品
- 别名
- hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody
- 背景
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Synonyms: BAC, Luciferase Biotin, Anti-Luciferase BAC Antibody
Background: Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract.
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