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Lactate Dehydrogenase A 抗体 (HRP)

Rockland 200-1373-0100 LDHA 适用: 兔 WB, ELISA, IHC, IF, IP 宿主: 山羊 Polyclonal HRP
Rockland
产品编号 ABIN5596934
Supplier Product No.: 200-1373-0100
发货至: 中国
  • 抗原 See all Lactate Dehydrogenase A (LDHA) 抗体
    Lactate Dehydrogenase A (LDHA)
    适用
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    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This Lactate Dehydrogenase A antibody is conjugated to HRP
    应用范围
    • 80
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Supplier Product No.
    200-1373-0100
    Supplier
    Rockland
    原理
    Lactate Dehydrogenase Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
    产品特性
    Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
    纯化方法
    Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.

    Immunogen Type: Native Protein

    亚型
    IgG
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    Discover our top product LDHA Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:1,000 - 1:6,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:3,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    Lactate Dehydrogenase A (LDHA)
    别名
    LDHA (LDHA 产品)
    背景
    Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.
    基因ID
    100009107, 126050
    UniProt
    P13491
    途径
    Warburg Effect
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