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- 抗原 See all CA2 抗体
- CA2 (Carbonic Anhydrase II (CA2))
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适用
- 人, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CA2 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 交叉反应 （详细）
- Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
- 免疫原
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Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product CA2 Primary Antibody
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anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibody
CA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibodyCA2 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (C-Term) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Carbonic anhydrase II has been assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- CA2 (Carbonic Anhydrase II (CA2))
- 别名
- Carbonic Anhydrase II (CA2 产品)
- 背景
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Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody
Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Gene Name: CA2
- 基因ID
- 280740
- UniProt
- P00921
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