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GFP 抗体

Rockland 600-301-215m GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
Rockland
产品编号 ABIN5596892
Supplier Product No.: 600-301-215m
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    适用
    • 165
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    Aequorea victoria
    宿主
    • 62
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    • 34
    • 13
    • 9
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    小鼠
    克隆类型
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    单克隆
    标记
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    This GFP antibody is un-conjugated
    应用范围
    • 142
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)
    Supplier Product No.
    600-301-215m
    Supplier
    Rockland
    原理
    GFP Monoclonal Antibody
    交叉反应 (详细)
    Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.
    产品特性
    Synonyms: mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
    纯化方法
    GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.

    Immunogen Type: Recombinant Protein

    克隆位点
    9F9-F9
    亚型
    IgG1 kappa
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

    Western Blot Dilution: 1:3000 - 1:30,000

    ELISA Dilution: 1:80,000 - 1:500,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    别名
    GFP (GFP 产品)
    背景
    Background: Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescence Microscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
    UniProt
    P42212
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