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- 抗原 See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
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适用
- Aspergillus niger
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Glucose Oxidase antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB)
- 交叉反应 （详细）
- Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Anti-Glucose Oxidase (Sheep) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
- 免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Glucose Oxidase (GOD) antibody
GOD 适用: Aspergillus niger IEP 宿主: 山羊 Polyclonal unconjugated
anti-Glucose Oxidase (GOD) antibody (HRP)GOD 适用: Aspergillus niger ELISA, WB, IHC 宿主: 兔 Polyclonal HRP
anti-Glucose Oxidase (GOD) antibody (Biotin)GOD 适用: Aspergillus niger ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Glucose Oxidase (GOD) antibodyGOD 适用: Aspergillus niger ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glucose Oxidase (GOD) antibodyGOD 适用: Aspergillus niger ELISA, WB 宿主: 绵羊 Polyclonal unconjugated
anti-Glucose Oxidase (GOD) antibodyGOD 适用: Aspergillus niger ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Glucose Oxidase (sheep) antibody has been assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:40,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Glucose Oxidase (GOD)
- 别名
- Glucose Oxidase (GOD 产品)
- 别名
- LOC406081 antibody, GB19418 antibody, glucose oxidase antibody, LOC406081 antibody, AFUA_2G14840 antibody, SS1G_10949 antibody, AOR_1_952194 antibody, AOR_1_786174 antibody, AOR_1_358034 antibody, CC1G_15386 antibody, VDBG_09040 antibody, VDBG_06502 antibody, SCHCODRAFT_113971 antibody
- 背景
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Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
Background: Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Glucose Oxidase (Sheep) antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.
Gene Name: gox
- UniProt
- P13006
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