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Phosphothreonine 抗体 (HRP)

Rockland 600-403-263 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
Rockland
产品编号 ABIN5596878
Supplier Product No.: 600-403-263
发货至: 中国
  • 抗原 See all Phosphothreonine products
    Phosphothreonine
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Phosphothreonine antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Supplier Product No.
    600-403-263
    Supplier
    Rockland
    原理
    Phosphothreonine Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.
    产品特性
    Synonyms: Phospho-Threonine Antibody, Phospho-Threonine ELISA, HRP Phospho Threonine (pT) antibody, PhosphoThreonine (pT) polyclonal antibody
    纯化方法
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Phospho Site Specific affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

    Immunogen Type: Other

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Phospho Site Specific Threonine affinity purified antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine proteins in both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

    Western Blot Dilution: 1:500

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:1,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: None
    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Puttagunta, Tedeschi, Sória, Hervera, Lindner, Rathore, Gaub, Joshi, Nguyen, Schmandke, Laskowski, Boutillier, Bradke, Di Giovanni: "PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system." in: Nature communications, Vol. 5, pp. 3527, (2014) (PubMed).

  • 抗原
    Phosphothreonine
    Abstract
    Phosphothreonine 产品
    物质类
    Amino Acid
    背景
    Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
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