Phosphothreonine 抗体 (Biotin)
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- 抗原 See all Phosphothreonine products
- Phosphothreonine
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phosphothreonine antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Supplier Product No.
- 600-406-263
- Supplier
- Rockland
- 原理
- Phosphothreonine Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.
- 产品特性
- Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit
- 纯化方法
- Anti-PhosphoThreonine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.
Immunogen Type: Other
- 亚型
- IgG
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anti-Phosphothreonine antibody
WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 594)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 488)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 390)WB, ELISA, IP, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine antibody (HRP)WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: PhosphoThreonine affinity-purified antibody is tested for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store anti-pT antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Phosphothreonine
- Abstract
- Phosphothreonine 产品
- 物质类
- Amino Acid
- 背景
- Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
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