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- 抗原 See all Phosphothreonine products
- Phosphothreonine
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抗原表位
- pThr
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phosphothreonine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.
- 纯化方法
- Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.
- 免疫原
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Immunogen: PhosphoThreonine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.
Immunogen Type: Other
- 亚型
- IgG
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anti-Phosphothreonine antibody
WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 488)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 594)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 390)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine (phosphorylated) antibody (HRP)WB, IP, ELISA, IHC 宿主: 兔 Polyclonal HRP
anti-Phosphothreonine antibodyIP, ELISA, IHC, FACS, RIA 宿主: 小鼠 Monoclonal 18F6 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Western Blot Dilution: 2.0 μg/mL
Immunoprecipitation Dilution: 10.0 μg/mg sample
ELISA Dilution: 0.5 μg/mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Phosphothreonine
- Abstract
- Phosphothreonine 产品
- 物质类
- Amino Acid
- 背景
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Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit
Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
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