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Esterase 抗体 (HRP)

适用: Pig ELISA, WB, IHC 宿主: 兔 Polyclonal HRP
产品编号 ABIN5596851
发货至: 中国
  • 抗原 See all Esterase products
    Esterase
    适用
    Pig
    宿主
    • 6
    克隆类型
    • 6
    多克隆
    标记
    • 3
    • 3
    This Esterase antibody is conjugated to HRP
    应用范围
    • 6
    • 6
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    交叉反应 (详细)
    Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
    纯化方法
    Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
    免疫原

    Immunogen: Esterase [Porcine Liver]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Esterase has been assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:250 - 1:1,000

    ELISA Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Esterase
    Abstract
    Esterase 产品
    别名
    Carboxylic ester hydrolase antibody, T28C12.4 antibody, K11G9.1 antibody, K07C11.4 antibody, F15A8.6 antibody, K11G9.2 antibody, K11G9.3 antibody, T28C12.5 antibody
    背景

    Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase

    Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.

    Gene Name: CES3

    基因ID
    100626873
    UniProt
    Q29550
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