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- 抗原 See all Esterase products
- Esterase
- 适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Esterase antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
- 免疫原
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Esterase antibody (Biotin)
适用: Pig ELISA, WB 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Esterase has been assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Esterase
- Abstract
- Esterase 产品
- 别名
- Carboxylic ester hydrolase antibody, T28C12.4 antibody, K11G9.1 antibody, K07C11.4 antibody, F15A8.6 antibody, K11G9.2 antibody, K11G9.3 antibody, T28C12.5 antibody
- 背景
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Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase
Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.
Gene Name: CES3
- 基因ID
- 100626873
- UniProt
- Q29550
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