(5 references)-
- 抗原 See all His Tag 抗体
- His Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This His Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)
- 序列
- HHHHHH
- 特异性
- Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
- 灵敏度
- WB (with ECL): 1-2ng of His-tagged fusion proteins
- 纯化方法
- Protein A affinity chromatography from mouse ascites fluid
- 免疫原
- HHHHHH (6x His) synthetic peptide conjugated to KLH
- 克隆位点
- HIS-H8
- 亚型
- IgG2b
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP
anti-His Tag (C-Term) antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody (Biotin)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin
anti-His Tag antibody (DyLight 649)WB, FLISA, FM 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649
anti-His Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
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- 应用备注
- WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- 限制
- 仅限研究用
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- 浓度
- 1mg/mL
- 缓冲液
- 10mM PBS, pH 7.2, 0.05% NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).
: "Vimentin and PSF act in concert to regulate IbeA+ E. coli K1 induced activation and nuclear translocation of NF-κB in human brain endothelial cells." in: PLoS ONE, Vol. 7, Issue 4, pp. e35862, (2012) (PubMed).
: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).
: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, (1998) (PubMed).
: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, (1998) (PubMed).
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: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).
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