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COL2A1 抗体

Name: COL2A1 抗体
SKU: ABIN5596824
Availability: OutOfStock
Rating: 5 (1 reviews)

Rockland 600-401-104-0.1 COL2A1 适用: 人, Cow WB, IHC, ELISA, IP, FM, DB 宿主: 兔 Polyclonal unconjugated

anti collagen II antibody (600-401-104 Lot 26014, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). (COL2A1 抗体)

IHC

2 images

(1 validation)

产品编号 ABIN5596824

Supplier Product No.: 600-401-104-0.1

发货至: 中国

Datasheet as PDF

抗原 See all COL2A1 抗体

COL2A1 (Collagen, Type II, alpha 1 (COL2A1))
适用
75

37

35

19

8

2

2

1

1

1

1
人, Cow
宿主
76

11

2

1
兔
Compatible Secondaries
克隆类型
80

10
多克隆
标记
46

7

6

5

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This COL2A1 antibody is un-conjugated
应用范围
61

32

25

13

13

13

13

12

10

10

9

5

3

3

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.

600-401-104-0.1

Supplier

Rockland

原理

Collagen Type II Antibody

交叉反应（详细）

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

产品特性

Synonyms: rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

纯化方法

Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

过滤

Sterile filtered

免疫原

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.
Immunogen Type: Native Protein

亚型

IgG

Top Product

Discover our top product COL2A1 Primary Antibody
anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1307-1383) antibody
COL2A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 873-1072) antibody
COL2A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibody
COL2A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1222-1241), (C-Term) antibody
COL2A1 适用: 小鼠, 大鼠 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 231-330) antibody
COL2A1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (C-Term) antibody
COL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (N-Term) antibody
COL2A1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1143-1297) antibody
COL2A1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) (AA 1228-1487) antibody
COL2A1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type II, alpha 1 (COL2A1) antibody
COL2A1 适用: 小鼠, 大鼠 IHC 宿主: 小鼠 Monoclonal 2F12D3 unconjugated

应用备注

Immunohistochemistry Dilution: 1:50 - 1:400
Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:50,000
IF Microscopy Dilution: User Optimized
Other: User Optimized

限制

仅限研究用
生效 #104083 (Unfolding Profile)

by

NanoTemper Technologies

No.

#104083

日期

2019.07.23

抗原

COL2

Lot Number

41639

Method validated

Unfolding Profile

Positive Control

ABIN5596824

Negative Control

Notes

Passed. ABIN5596824 showed T_i at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.
Full Methods
Primary Antibody

Secondary Antibody

Full Protocol

Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
Run Tycho measurement.

Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

有效期

12 months
抗原

COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

别名

COL2A1 (COL2A1 产品)

背景

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

基因ID

1280

NCBI登录号

NP_001835

UniProt

P02458

途径

Sensory Perception of Sound, Growth Factor Binding