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CD20 抗体 (FITC)

Name: CD20 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN559682
Availability: OutOfStock

MS4A1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 2H7 FITC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD20 (2H7) FITC antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD20 抗体 (FITC))

FACS

Surface staining of human peripheral blood with anti-CD20 (2H7) FITC. (CD20 抗体 (FITC))

FACS

Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD20 (2H7) FITC antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD20 抗体 (FITC))

FACS

3 images

(7 references)

产品编号 ABIN559682

发货至: 中国

Datasheet as PDF

抗原 See all CD20 (MS4A1) 抗体

CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
适用
251

63

28

19

18

17

11

6

6

4

2

1
人, Non-Human Primate
宿主
179

98

4

2
小鼠
Compatible Secondaries
克隆类型
198

83

1
单克隆
标记
108

21

17

17

13

7

6

6

6

6

6

6

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD20 antibody is conjugated to FITC
应用范围
206

92

77

52

47

33

25

18

16

16

14

13

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11

6

5

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2

1

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Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody 2H7 recognizes an extracellular epitope on CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human tonsillar B cells

克隆位点

2H7

亚型

IgG2b kappa

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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibody
MS4A1 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody
Verified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal L26-IGEL-773 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
MS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
MS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Hayden-Ledbetter, Cerveny, Espling, Brady, Grosmaire, Tan, Bader, Slater, Nilsson, Barone, Simon, Bradley, Thompson, Wahl, Ledbetter: "CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2739-46, (2009) (PubMed).

Diehl, Schmidlin, Nagasawa, van Haren, Kwakkenbos, Yasuda, Beaumont, Scheeren, Spits: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, (2008) (PubMed).

Polyak, Ayer, Szczepek, Deans: "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody." in: Leukemia, Vol. 17, Issue 7, pp. 1384-9, (2003) (PubMed).

Chan, Hughes, French, Tutt, Walshe, Teeling, Glennie, Cragg: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, (2003) (PubMed).

Rose, Smith, Maloney: "Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects." in: Blood, Vol. 100, Issue 5, pp. 1765-73, (2002) (PubMed).

Deans, Robbins, Polyak, Savage: "Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 344-8, (1998) (PubMed).

Deans, Kalt, Ledbetter, Schieven, Bolen, Johnson: "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22632-8, (1995) (PubMed).
抗原

CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

别名

CD20 (MS4A1 产品)

别名

B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody

背景

MS4A1,CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.,B1, S7, MS4A, Bp35, CVID5, LEU-16

基因ID

931

UniProt

P11836