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beta-2 Microglobulin 抗体 (HRP)

Rockland 200-403-141-0100 B2M 适用: 人 WB, ELISA, IHC, FM 宿主: 兔 Polyclonal HRP
Rockland
产品编号 ABIN5596817
Supplier Product No.: 200-403-141-0100
发货至: 中国
  • 抗原 See all beta-2 Microglobulin (B2M) 抗体
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    适用
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    This beta-2 Microglobulin antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Supplier Product No.
    200-403-141-0100
    Supplier
    Rockland
    原理
    Beta-2-Microglobulin Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine).
    产品特性
    Synonyms: rabbit anti-Beta-2-Microglobulin Antibody Peroxidase Conjugation, HRP conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody
    纯化方法
    Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product B2M Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-beta-2-Microglobulin Peroxidase antibody has been tested in ELISA and western blotting, and is suitable for IF and IHC. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:2,000 - 1:10,000

    IF Microscopy Dilution: 1:200

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    别名
    B2M (B2M 产品)
    背景
    Background: Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.
    基因ID
    567
    NCBI登录号
    NP_004039
    UniProt
    P61769
    途径
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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