PIPOX 抗体 (HRP)
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- 抗原 See all PIPOX 抗体
- PIPOX (Pipecolic Acid Oxidase (PIPOX))
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适用
- Microbial
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This PIPOX antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应 (详细)
- Cross reactivity against Sarcosine Oxidase from other sources is unknown.
- 纯化方法
- Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].
- 免疫原
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Immunogen: Sarcosine Oxidase [Microbial]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product PIPOX Primary Antibody
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- 应用备注
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Application Note: Anti-Sarcosine Oxidase has been assayed against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- PIPOX (Pipecolic Acid Oxidase (PIPOX))
- 别名
- Sarcosine Oxidase (PIPOX 产品)
- 别名
- LPIPOX antibody, Pso antibody, SOX antibody, pipecolic acid and sarcosine oxidase antibody, pipecolic acid oxidase antibody, pipecolic acid oxidase L homeolog antibody, PIPOX antibody, Pipox antibody, pipox antibody, pipox.L antibody
- 背景
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Synonyms: Sarcosine oxidase subunit alpha Sarcosine oxidase subunit EC=1.5.3.1
Background: Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.
Gene Name: SOXA
- 基因ID
- 10586229
- UniProt
- Q46337
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