TNF alpha 抗体
(1 reference)
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- 抗原 See all TNF alpha 抗体
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TNF alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Supplier Product No.
- 209-401-306-0100
- Supplier
- Rockland
- 原理
- TNF alpha Antibody
- 交叉反应 （详细）
- This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFα in the same formats using similar dilutions.
- 产品特性
- Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:100 - 1:500
Application Note: Anti-TNF alpha antibody has been tested in WB, ELISA, IF, and IHC. Expect a band in appropriate lysates at ~25.6 kDa. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays diluted at 1:200. The antibody does not recognize human TNF beta (lymphotoxin). Specific conditions for reactivity and signal detection should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store anti-TNF alpha at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, (2011) (PubMed).
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: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, (2011) (PubMed).
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- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- TNF (TNF alpha 产品)
- 背景
- Background: Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD). Anti-TNF alpha Antibody is useful for researches interested in TNF/TNFR pathways, apoptosis signaling pathways, and cell proliferation.
- 基因ID
- 7124
- UniProt
- P01375
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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