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- 抗原 See all Streptavidin 抗体
- Streptavidin
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适用
- Streptomyces avidinii
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Streptavidin antibody is conjugated to HRP
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应用范围
- ELISA
- 交叉反应 （详细）
- No cross reactivity occurs against avidin.
- 纯化方法
- Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].
- 免疫原
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Immunogen: Streptavidin [Streptomyces avidinii]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Streptavidin Primary Antibody
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anti-Streptavidin antibody
适用: Streptomyces avidinii FACS, IM 宿主: 兔 Polyclonal unconjugated
anti-Streptavidin antibody (Texas Red (TR))适用: Streptomyces avidinii FACS, FLISA, FM 宿主: 兔 Polyclonal Texas Red (TR)
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, FLISA, FM 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody (Biotin)适用: Streptomyces avidinii ELISA 宿主: 兔 Polyclonal Biotin
anti-Streptavidin antibody适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: This product has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassyas should be determined by researcher.
ELISA Dilution: 1:2,000-1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
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Synonyms: HRP, Streptavidin Peroxidase, Anti-Streptavidin HRP Antibody
Background: Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^-14 Mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH .
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