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Pyruvate Kinase 抗体 (HRP)

PK 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal HRP
产品编号 ABIN5596779
发货至: 中国
  • 抗原 See all Pyruvate Kinase (PK) 抗体
    Pyruvate Kinase (PK)
    适用
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    宿主
    • 8
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    山羊
    克隆类型
    • 17
    多克隆
    标记
    • 14
    • 3
    This Pyruvate Kinase antibody is conjugated to HRP
    应用范围
    • 15
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    Western Blotting (WB), ELISA
    交叉反应 (详细)
    This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.
    纯化方法
    Pyruvate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].
    免疫原

    Immunogen: Pyruvate Kinase [Rabbit Muscle]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product PK Primary Antibody
  • 应用备注

    Application Note: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:4,000 - 1:20,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Pyruvate Kinase (PK)
    别名
    Pyruvate Kinase (PK 产品)
    别名
    CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, PYK3b antibody, PYK3c antibody, Pyruvate kinase antibody, PK antibody, pyruvate kinase antibody, pyruvate kinase (PK) antibody, Pyruvate kinase (PK) antibody, pyruvate kinase PKM antibody, testis expressed 45 antibody, PyK antibody, pykA antibody, PYK3a|PYK3b|PYK3c antibody, pyk antibody, pyk1 antibody, pk antibody, LOC100534543 antibody, TEX45 antibody
    背景

    Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, HRP

    Background: Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.

    Gene Name: PKM2

    基因ID
    100008676
    UniProt
    P11974
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