Fructosyl Amino Acid Oxidase 抗体 (HRP)
-
- 抗原 See all Fructosyl Amino Acid Oxidase (socD) products
- Fructosyl Amino Acid Oxidase (socD)
-
适用
- Corynebacterium
-
宿主
-
绵羊
-
克隆类型
- 多克隆
-
标记
- This Fructosyl Amino Acid Oxidase antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.
- 纯化方法
- Fructosyl-amino acid oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli].
- 免疫原
-
Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
Immunogen Type: Recombinant Protein
- 亚型
- IgG
-
-
- 应用备注
-
Application Note: Anti-Fructosyl-amino acid oxidase has been assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Fructosyl Amino Acid Oxidase (socD)
- 别名
- Fructosyl-Amino Acid Oxidase (socD 产品)
- 别名
- fructosyl amino acid oxidase antibody, hypothetical protein antibody, socD antibody, CNB00220 antibody, AFUA_8G06440 antibody, AFUA_6G03440 antibody, PTRG_01708 antibody, ANI_1_1464184 antibody, AOR_1_60064 antibody, PGTG_09157 antibody
- 背景
-
Synonyms: Fructosyl-amino acid oxidase EMBL BAB91123.1 EC=1.5.3
Background: Fructosyl-amino acid oxidase catalyzed the oxidation of fructosyl-amino acids to produce glucosone, hydrogen peroxide and amino acid. An enzyme that can be used for the determination of glycated proteins in blood.
Gene Name: faoxC
- UniProt
- Q8RIU8
-
