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Cholesterol Oxidase 抗体 (HRP)

choS 适用: Streptomyces ELISA, WB, IP 宿主: 山羊 Polyclonal HRP
产品编号 ABIN5596764
发货至: 中国
  • 抗原 See all Cholesterol Oxidase (choS) products
    Cholesterol Oxidase (choS)
    适用
    • 5
    • 4
    • 4
    • 1
    Streptomyces
    宿主
    • 12
    • 2
    山羊
    克隆类型
    • 14
    多克隆
    标记
    • 5
    • 5
    • 2
    • 2
    This Cholesterol Oxidase antibody is conjugated to HRP
    应用范围
    • 14
    • 10
    • 4
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    交叉反应 (详细)
    Cross reactivity against Cholesterol Oxidase from other sources is unknown.
    纯化方法
    Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].
    免疫原

    Immunogen: Cholesterol Oxidase [Streptomyces]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Cholesterol Oxidase has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Cholesterol Oxidase (choS)
    别名
    Cholesterol Oxidase (choS 产品)
    别名
    SCD63.13 antibody, cholesterol oxidase antibody, GMC family oxidoreductase antibody, Cholesterol oxidase antibody, choS antibody, SCO4781 antibody, BTH_I0619 antibody, SARE_RS13770 antibody, Lcho_3191 antibody, Npun_R6526 antibody, Amir_2425 antibody, Caci_5961 antibody, Sros_4246 antibody, Kfla_6484 antibody, Snas_3535 antibody, Weevi_2091 antibody
    背景

    Synonyms: choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

    Background: Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

    Gene Name: choA

    基因ID
    1215660
    UniProt
    P12676
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