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- 抗原
- LMW HCP (LMW Host Cell Proteins (LMW HCP))
- 适用
- E. coli
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)
- 原理
- Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli.
- 产品特性
- Synonyms: rabbit anti-E.Coli Host Cell Protein antibody, rabbit anti-HCP antibody, rabbit anti-Low molecular Weight E.Coli Host Cell Proteins Antibody, Anti-Host Cell Protein, Low molecular weight E.Coli HCP proteins antibody
- 纯化方法
- LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-LMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).
Immunogen Type: Native Protein
- 亚型
- IgG
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- 应用备注
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Application Note: Anti-LMW Host Cell Proteins Antibody detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA.
Western Blot Dilution: 1:500-1:1000
ELISA Dilution: 1:1000 - 1:2000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.29 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- LMW HCP (LMW Host Cell Proteins (LMW HCP))
- 别名
- LMW E.Coli Host Cell Protein
- 背景
- Background: Anti-LMW Host Cell Proteins Antibody recognizes low molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
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