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- 抗原
- HMW HCP (HMW Host Cell Proteins (HMW HCP))
- 适用
- E. coli
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB)
- 原理
- Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.
- 特异性
- HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins.
- 产品特性
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Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP
Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
- 纯化方法
- HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli.
Immunogen Type: Native Protein
- 亚型
- IgG
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- 应用备注
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Western Blot Dilution: 1:500 - 1:1000
ELISA Dilution: 1:1000-1:2000
- 说明
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Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.38 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- HMW HCP (HMW Host Cell Proteins (HMW HCP))
- 别名
- HMW E.Coli Host Cell Proteins
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