Hexokinase 抗体 (HRP)
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- 抗原 See all Hexokinase 抗体
- Hexokinase
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hexokinase antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 (详细)
- Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.
- 纯化方法
- Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 免疫原
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Immunogen: Hexokinase [Yeast]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Hexokinase Primary Antibody
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- 应用备注
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Application Note: Anti-HEXOKINASE (RABBIT) Antibody can be used in applications such as Immunohistochemistry (IHC), Western blot (WB) Immunofluorescence (IF) ELISA. Specific conditions should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:1,000
ELISA Dilution: 1:1,000 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Hexokinase
- Abstract
- Hexokinase 产品
- 背景
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Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB
Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.
Gene Name: HXK2
- 基因ID
- 852639
- UniProt
- P04807
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