Hexokinase 2 抗体 (HRP)
-
- 抗原 See all Hexokinase 2 (HK2) 抗体
- Hexokinase 2 (HK2)
-
适用
- Saccharomyces cerevisiae
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Hexokinase 2 antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-4359-0100
- Supplier
- Rockland
- 原理
- Hexokinase Antibody Peroxidase Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 产品特性
- Synonyms: rabbit anti-Hexokinase antibody HRP conjugation, peroxidase conjugation rabbit anti-Hexokinase antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody, HEX1, HKB
- 纯化方法
- Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Hexokinase [Yeast]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product HK2 Primary Antibody
-
-
- 应用备注
-
Application Note: Anti-HEXOKINASE Peroxidase Conjugated Antibody has been tested by ELISA and western blot and can be used in applications such as Immunohistochemistry (IHC), and Immunofluorescence (IF). Specific conditions should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:1,000
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Hexokinase 2 (HK2)
- 别名
- HXK2 (HK2 产品)
- 背景
- Background: HEXOKINASE Antibody is specific for hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose. Anti Hexokinase is ideal for investigators involved in glucose energy metabolism, enzymes and AKT signaling research.
- 基因ID
- 852639
- UniProt
- P04806
- 途径
- PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect
-