Dextranase 抗体 (HRP)
-
- 抗原 See all Dextranase products
- Dextranase
- 适用
- Penicillium sp.
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Dextranase antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Dextranase from other sources is unknown.
- 纯化方法
- Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
- 免疫原
-
Immunogen: Dextranase [Penicillium species]
Immunogen Type: Native Protein
- 亚型
- IgG
-
anti-Dextranase antibody
适用: Penicillium sp. ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: Anti-Dextranase has been assayed against 1.0 μg of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Dextranase
- Abstract
- Dextranase 产品
- 别名
- dextranase antibody, Dextranase antibody, Teth39_0264 antibody, Teth514_0757 antibody, VDBG_04792 antibody, Thet_2161 antibody, Thebr_0271 antibody
- 背景
-
Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX
Background: Dextranase belongs to the glycosyl hydrolase 49 family. It's catalytic reaction is endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
Gene Name: DEX
- UniProt
- P48845
-
