Calf Intestinal Alkaline Phosphatase (CIAP) 抗体 (HRP)
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- 抗原 See all Calf Intestinal Alkaline Phosphatase (CIAP) products
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-4335-0100
- Supplier
- Rockland
- 原理
- Alkaline Phosphatase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
- 产品特性
- Synonyms: rabbit anti-Alkaline Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- 纯化方法
- Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody
CIAP 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody (Biotin)CIAP 适用: Cow WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Alkaline phosphatase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is assayed against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 别名
- Calf Intenstinal Alkaline Phosphatase (CIAP 产品)
- 背景
- Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
- 基因ID
- 280993
- UniProt
- P19111
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