Calf Intestinal Alkaline Phosphatase (CIAP) 抗体 (HRP)
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- 抗原 See all Calf Intestinal Alkaline Phosphatase (CIAP) products
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 200-1335-0100
- Supplier
- Rockland
- 原理
- Alkaline Phosphatase Antibody HRP Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
- 产品特性
- Synonyms: goat anti-Alkaline Phosphatase Antibody HRP Conjugation, Peroxidase Conjugation goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- 纯化方法
- Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody
CIAP 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody (Biotin)CIAP 适用: Cow WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 别名
- Calf Intenstinal Alkaline Phosphatase (CIAP 产品)
- 背景
- Background: Alkaline Phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. Humans and most other mammals contain the following alkaline phosphatase isozymes: ALPI - intestinal, ALPL - tissue non-specific (liver/bone/kidney), ALPP - placental (Regan isozyme). Elevated ALP indicates that there could be active bone formation occurring as ALP is a byproduct of osteoblast activity (such as the case in Paget's disease of bone). Levels are also elevated in people with untreated Coeliac Disease. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
- 基因ID
- 280993
- UniProt
- P19111
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