Aldehyde Dehydrogenase 抗体 (HRP)
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- 抗原 See all Aldehyde Dehydrogenase (ALDH) 抗体
- Aldehyde Dehydrogenase (ALDH)
- 适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Aldehyde Dehydrogenase antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 (详细)
- Cross reactivity against Aldehyde Dehydrogenase from other sources is unknown.
- 纯化方法
- Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].
- 免疫原
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Immunogen: Aldehyde Dehydrogenase [Yeast]
Immunogen Type: Native Protein
- 亚型
- IgG
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- 应用备注
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Application Note: Anti-Aldehyde dehydrogenase has been assayed against 1.0 μg of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Aldehyde Dehydrogenase (ALDH)
- 别名
- Aldehyde Dehydrogenase (ALDH 产品)
- 别名
- 146084_at antibody, ACDH antibody, ALDH antibody, CG 3752 antibody, CG3752 antibody, Dmel\\CG3752 antibody, GB12741 antibody, Tb06.26G9.1140 antibody, Aldehyde dehydrogenase antibody, ALdehyde deHydrogenase antibody, aldehyde dehydrogenase, mitochondrial antibody, aldehyde dehydrogenase antibody, betaine-aldehyde dehydrogenase antibody, ran-specific GTPase-activating protein antibody, Aldh antibody, alh-6 antibody, alh-12 antibody, LOC550687 antibody, aldH antibody, Tb927.6.4210 antibody, HQ_RS05000 antibody, aldA-2 antibody, ALDH antibody, LOC618976 antibody
- 背景
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Synonyms: Aldehyde dehydrogenase [NAD(P)+] 1 ALD2, ALD5
Background: Aldehyde dehydrogenase are a group of enzymes that catalyse the oxidation (dehydrogenation) of aldehydes. Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. ALDH2 also plays a crucial role in maintaining low blood levels of acetaldehyde during alcohol oxidation.
Gene Name: ALD2, ALD5
- 基因ID
- 855206
- UniProt
- P47771
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