Recombinant POLR2F 抗体
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- 抗原 See all POLR2F 抗体
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This POLR2F antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)
- 品牌
- AbFlex®
- 产品特性
- AbFlex™ RNA Pol II antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
- 纯化方法
- Protein A
- 亚型
- IgG2a
- Top Product
- Discover our top product POLR2F Primary Antibody
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibody
POLR2F 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人 WB, IF, ChIP, ChIP-seq, ICC 宿主: 大鼠 Monoclonal 1F4B6 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (N-Term) antibodyPOLR2F 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠, 小鼠, C. elegans, Saccharomyces cerevisiae WB, IF, ChIP, ChIP-seq, ICC 宿主: 小鼠 Monoclonal 4H8 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 27-61) antibodyPOLR2F 适用: 小鼠 WB 宿主: 兔 Polyclonal RB56843 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) antibodyPOLR2F 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F) (AA 1-127) antibodyPOLR2F 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- ChIP-Seq: 4 μg per ChIP Western Blot: 0.1 - 1 μg/mL Immunofluorescence (IF): 2 μg/mL.
- 说明
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))
- 别名
- RNA Pol II (POLR2F 产品)
- 别名
- HRBP14.4 antibody, POLRF antibody, RPABC2 antibody, RPB14.4 antibody, RPB6 antibody, 1810060D16Rik antibody, RNA polymerase II subunit F antibody, polymerase (RNA) II (DNA directed) polypeptide F antibody, POLR2F antibody, Polr2f antibody
- 分子量
- 217 kDa
- 途径
- Regulatory RNA Pathways
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