Recombinant H2AFV 抗体
-
- 抗原 See all H2AFV 抗体
- H2AFV (H2A Histone Family, Member V (H2AFV))
- 抗体类型
- Recombinant Antibody
-
适用
- Drosophila melanogaster
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This H2AFV antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
- 品牌
- AbFlex®
- 特异性
- Drosophila
- 产品特性
- AbFlex™ Histone H2Av antibody (biotin) was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody was directly labeled with biotin using the biotin ligase, BirA. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.
- 纯化方法
- Ni-NTA
- 亚型
- IgG2a
- Top Product
- Discover our top product H2AFV Primary Antibody
-
anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibody
H2AFV 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibodyH2AFV 适用: 人 ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (C-Term) antibodyH2AFV 适用: Drosophila melanogaster WB, ChIP 宿主: 小鼠 Monoclonal 10E9-D1 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 3-128) antibodyH2AFV 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (N-Term) antibodyH2AFV 适用: 人, Drosophila melanogaster, 豚鼠, 斑马鱼, 马, 小鼠, 兔, 大鼠, Cow, 绵羊, 犬, 猴, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-129) antibodyH2AFV 适用: 人 ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
-
- 应用备注
- ChIP: 10 μg per ChIP WB: 0.5 - 2 μg/mL dilution Bead-based ELISA: 7 - 560 ng/mL
- 说明
-
AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Purified IgG in 50 mM sodium phosphate pH 8.0, 150 mM NaCl, and 100 mM imidazole with 30 % glycerol and 0.035 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
-
- 抗原
- H2AFV (H2A Histone Family, Member V (H2AFV))
- 别名
- Histone H2Av (H2AFV 产品)
- 别名
- H2A.Z-2 antibody, H2afv antibody, H2AV antibody, h2av antibody, C530002L11Rik antibody, H2a.z-2 antibody, H2av antibody, H2A.F/Z antibody, h2afv antibody, h2afz antibody, H2A histone family, member V antibody, H2A histone family member V antibody, H2A histone family member V S homeolog antibody, H2A histone family, member Va antibody, H2afv antibody, LOC100226437 antibody, H2AFV antibody, h2afv.S antibody, h2afva antibody
- 分子量
- 15 kDa
-
