CRISPR-Cas9 抗体
-
- 抗原
- CRISPR-Cas9
-
适用
- Streptococcus pyogenes
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB)
- 纯化方法
- Affinity Purified
- 克隆位点
- 11E7-
- 亚型
- IgG2a
-
anti-CRISPR-Cas9 (AA 1-462) antibody
适用: Staphylococcus aureus WB 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-CRISPR-Cas9 (C-Term) antibody适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 antibody适用: Staphylococcus aureus WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (HRP)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal HRP
anti-CRISPR-Cas9 (C-Term) antibody (Biotin)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal Biotin
anti-CRISPR-Cas9 antibody适用: Streptococcus pyogenes WB, IF, IP, ChIP 宿主: 小鼠 Monoclonal 8C1-F10 unconjugated
anti-CRISPR-Cas9 antibody适用: 人 WB 宿主: 小鼠 Monoclonal 1E8 unconjugated
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 800)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 800
anti-CRISPR-Cas9 (C-Term) antibody (DyLight 549)适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal DyLight 549
-
- 应用备注
- Validated Applications: WB: 0.5 - 2 μg/mL
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
-
- 抗原
- CRISPR-Cas9
- 别名
- Cas9
- 背景
- SaCas9 is a nuclease from Staphylococcus aureus that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
- 分子量
- 130 kDa
-
