NeuN 抗体 (AA 1-140)
(2 validations)-
- 抗原 See all NeuN (RBFOX3) 抗体
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
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抗原表位
- AA 1-140
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NeuN antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- RBFOX3 Antibody
- 纯化方法
- Purified antibody
- 免疫原
- Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli.
- 克隆位点
- 1H3B2
- 亚型
- IgG2a
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anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-220) antibody
RBFOX3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibodyRBFOX3 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5A4B9 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 51-150) antibodyRBFOX3 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-101) antibodyRBFOX3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyMicroarray verified RBFOX3 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB503575 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (C-Term) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000
- 限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104496
- 日期
- 2023.08.02
- 抗原
- RBFOX3
- Lot Number
- 160425
- Method validated
- Western Blotting
- Positive Control
Adult mouse brain and cerebellum
- Negative Control
- Notes
The NeuN antibody ABIN5542704 works in WB at a 1:1000 dilution with sensitive ECL substrate on adult mouse brain and cerebellum extracts. Non-specific bands are also detected.
- Primary Antibody
- ABIN5542704
- Secondary Antibody
HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS)
- Full Protocol
- Homogenize tissues with cold lysis buffer containing 50 mM Tris HCl, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1% protease inhibitor (Sigma P8340) using an ultrasonic homogenizer (MSE, SoniPrep 150) with 16 amplitude, 20 s on, 10 s off pulse for 60 s.
- Centrifuge tissue homogenates at 13,000 rpm for 20 min at 4 °C.
- Collect supernatants and determine total protein content using a Bradford assay.
- Denature 50 µg of total protein for 5 min at 90 °C and subsequently separate them on a denaturing 12% PAGE-SDS gel alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, 160374).
- Electro-transfer proteins onto nitrocellulose membrane (Amersham Biosciences, RPN203D) ON in the cold room.
- Wash membrane 3x for 10 min with 0.01 M PBS containing 0.1% Tween-20 (PBST).
- Block membrane with PBST containing 2% bovine serum albumin for 1 h at RT.
- Incubate membrane with primary mouse anti-NeuN antibody (antibodies-online, ABIN5542704, lot 160425) diluted 1:1,000 in PBST ON at 4 °C.
- Wash membrane 3x 10 min with PBST.
- Incubate membrane with secondary HRP-conjugated goat anti-mouse IgG (Sigma-Aldrich, NA931VS) diluted 1:1,000 in PBST for 1 h at RT.
- Wash membrane 3x 10 min with PBST.
- Visualize proteins with WesternBright Sirius HRP substrate (Advansta) using a ChemiDoc Imaging System.
- Experimental Notes
Non-specific bands were also detected in adult mouse cerebellum tissue extracts, but not on laser-microdissected Purkinje neurons.
生效 #104496 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot using ABIN5542704 to reveal CALB1 (28 kDa) in extracts from two different mouse brains (1 and 2) and in cerebellum extracts (3).]()
Western blot using ABIN5542704 to reveal CALB1 (28 kDa) in extracts from two different mouse brains (1 and 2) and in cerebellum extracts (3).
Full Methods -
- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104533
- 日期
- 2024.01.26
- 抗原
- NeuN
- Lot Number
- 160425
- Method validated
- Immunohistochemistry
- Positive Control
Adult (> 2 months) CD1 mouse brain fixed in 4% paraformaldehyde.
- Negative Control
One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.
- Notes
Passed. NeuN antibody (AA 1-140) ABIN5542704 works in for IHC-P at 1:100 concentration with microwave antigen retrieval.
- Primary Antibody
- ABIN5542704
- Secondary Antibody
- anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher A11037, lot # 2439060)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix of samples in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Wash several times with 0.01 M PBS.
- Cut brain with a microtome into 6 µm sections and mount on glass slides.
- After paraffin removal sections were processed for microwave antigen retrieval for 10 minutes (95-100° C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 minutes each; followed by PBS for 5 minutes).
- Incubate sections for 1 h at RT in a humid chamber in PBS containing 1% ovalbumin (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary rabbit NeuN antibody (AA 1-140) (antibodies-online, ABIN5542704, lot 160425) diluted 1:50 and 1:100 in PBS-BSA-PLL (Sigma, P1524) ON at RT in a humid chamber.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with anti-rabbit secondary antibody Alexa Fluor 594, 1:500, in 0.1M PBS (Thermo Fisher A11037, lot # 2439060) for 1 h at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V.
- Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 40x magnification.
- Experimental Notes
生效 #104533 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Neu-N immunoreactive neuronal nuclei in the mouse cerebral cortex. The area in the rectangle is shown at higher magnification in the right panel. Original magnification 20x – insert 40x.]()
Neu-N immunoreactive neuronal nuclei in the mouse cerebral cortex. The area in the rectangle is shown at higher magnification in the right panel. Original magnification 20x – insert 40x.
Full Methods -
- 状态
- Liquid
- 缓冲液
- Purified antibody in PBS with 0.05 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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- 抗原
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- 别名
- RBFOX3 (RBFOX3 产品)
- 别名
- FOX-3 antibody, FOX3 antibody, HRNBP3 antibody, NEUN antibody, Fox-3 antibody, Hrnbp3 antibody, NeuN antibody, Neuna60 antibody, Neun antibody, RGD1560070 antibody, RNA binding fox-1 homolog 3 antibody, RNA binding protein, fox-1 homolog (C. elegans) 3 antibody, RNA binding protein, fox-1 homolog 3 antibody, RBFOX3 antibody, Rbfox3 antibody
- 背景
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Description: RBFOX3 (RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3) is a Protein Coding gene. Diseases associated with RBFOX3 include vertebral artery occlusion and tuberculum sellae meningioma. Among its related pathways are Neuroscience. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBFOX1.
Aliases: FOX3, NEUN, FOX-3, HRNBP3
- 分子量
- 33.8kDa
- 基因ID
- 146713
- HGNC
- 23492
- UniProt
- A6NFN3
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