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IDH1 抗体 (AA 156-298)

Name: IDH1 抗体 (AA 156-298)
Brand: antibodies-online
SKU: ABIN5542624
Availability: OutOfStock

IDH1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4A4A8 unconjugated

Western blot analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (IDH1 抗体 (AA 156-298))

Flow cytometric analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red). (IDH1 抗体 (AA 156-298))

FACS

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) (IDH1 抗体 (AA 156-298))

ELISA

Western blot analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate. (IDH1 抗体 (AA 156-298))

Western blot analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate. (IDH1 抗体 (AA 156-298))

5 images

产品编号 ABIN5542624

发货至: 中国

Datasheet as PDF

抗原 See all IDH1 抗体

IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
抗原表位
15

12

9

5

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 156-298
适用
97

58

41

7

7

5

5

5

5

4

4

4

3

2

2

1

1
人
宿主
78

35

3

1
小鼠
Compatible Secondaries
克隆类型
79

38
单克隆
标记
62

10

9

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This IDH1 antibody is un-conjugated
应用范围
83

34

30

28

20

16

14

13

9

6

5

2

2

2

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS)

原理

IDH1 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.

克隆位点

4A4A8

亚型

IgG1

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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibody
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 63-90) antibody
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 74-333) antibody
IDH1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

别名

IDH1 (IDH1 产品)

别名

IDCD antibody, IDH antibody, IDP antibody, IDPC antibody, PICD antibody, NADP-CICDH antibody, AI314845 antibody, AI788952 antibody, E030024J03Rik antibody, Id-1 antibody, Idh-1 antibody, Idpc antibody, cb876 antibody, fm90e09 antibody, im:7143416 antibody, wu:fm90e09 antibody, F23E12.180 antibody, F23E12_180 antibody, IDH-I antibody, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 antibody, isocitrate dehydrogenase 1 antibody, isocitrate dehydrogenase I antibody, isocitrate dehydrogenase (NADP(+)) 1, cytosolic antibody, isocitrate dehydrogenase 1 (NADP+), soluble antibody, isocitrate dehydrogenase 1 (NADP+) L homeolog antibody, isocitrate dehydrogenase 1 antibody, IDH1 antibody, Idh1 antibody, idh1.L antibody, idh1 antibody

背景

Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Aliases: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26

分子量

46.7kDa

基因ID

3417

HGNC

10393

UniProt

O75874

途径

Warburg Effect