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TLR9 抗体 (AA 868-1016)

Name: TLR9 抗体 (AA 868-1016)
Brand: antibodies-online
SKU: ABIN5542492
Availability: OutOfStock
Rating: 5 (2 reviews)

TLR9 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1B12H2 unconjugated

Immunofluorescence validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492) (TLR9 抗体 (AA 868-1016))

Proximity Ligation Assay validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492) (TLR9 抗体 (AA 868-1016))

PLA

Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein. (TLR9 抗体 (AA 868-1016))

Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red). (TLR9 抗体 (AA 868-1016))

9 images

(2 validations)

产品编号 ABIN5542492

发货至: 中国

Datasheet as PDF

抗原 See all TLR9 抗体

TLR9 (Toll-Like Receptor 9 (TLR9))
抗原表位
18

15

8

6

6

5

4

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 868-1016
适用
92

38

11

7

6

3

3

2

2

2

2

2

2

1

1
人
宿主
61

31

8

7

1
小鼠
Compatible Secondaries
克隆类型
70

38
单克隆
标记
59

13

13

8

5

4

1

1

1

1

1

1
This TLR9 antibody is un-conjugated
应用范围
71

46

44

42

22

14

10

9

6

5

4

3

2

2

2

2

1
Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

原理

TLR9 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. Coli.

克隆位点

1B12H2

亚型

IgG2a
anti-Toll-Like Receptor 9 (TLR9) (AA 28-362) antibody
TLR9 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 9 (TLR9) (AA 350-450) antibody
TLR9 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 9 (TLR9) (AA 26-193) antibody
TLR9 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5B10E8 unconjugated

anti-Toll-Like Receptor 9 (TLR9) (AA 951-1032) antibody
TLR9 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 9 (TLR9) (AA 99-215) antibody
TLR9 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E8 unconjugated

anti-Toll-Like Receptor 9 (TLR9) antibody (Biotin)
TLR9 适用: 人 WB, FACS, IHC (p), IF 宿主: 小鼠 Monoclonal 5G5 Biotin

anti-Toll-Like Receptor 9 (TLR9) (N-Term) antibody
TLR9 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 9 (TLR9) antibody
TLR9 适用: 人 WB, FACS, IHC (p), IF 宿主: 小鼠 Monoclonal 5G5 unconjugated

anti-Toll-Like Receptor 9 (TLR9) antibody (FITC)
TLR9 适用: 人 WB, FACS, IHC (p), IF 宿主: 小鼠 Monoclonal 5G5 FITC

anti-Toll-Like Receptor 9 (TLR9) (AA 26-332) antibody
TLR9 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

限制

仅限研究用
生效 #103693 (Immunofluorescence)

by

Klinik für Anästhesie, Intensivmedizin und Schmerztherapie, Universitätsklinikum der Ruhr Universität Bochum

No.

#103693

日期

2019.03.26

抗原

TLR9

Lot Number

160127

Method validated

Immunofluorescence

Positive Control

U937 monocytic cells

Negative Control

No primary antibody control

Notes

Passed. ABIN5542492 localization on ER is visible. Negative control without primary antibody shows no signal.

Validation Images

U937 monocytic cells stained with with ABIN5542492 (top row). TLR9 staining (AF488, green) shows typical peri-nuclear localization (ER). Counterstain with DAPI (blue). Negative control without primary antibody (bottom row) shows no significant staining. Counterstain with DAPI (blue).
Full Methods
Primary Antibody

ABIN5542492

Secondary Antibody

AF488-conjugated AffiniPure alpaca anti-Mouse IgG antibody (Jackson ImmunoResearch, 615-545-214)

Full Protocol

Grow U937 cells in RPMI (Gibco, 21875-034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep(Gibco, 15140-122), at 37°C and 5% CO₂ in 500µL in 12-well plates.
Use cytospin to spin cells onto slide.
Fix cells on coverslide in 4% PFA for 20min at RT.
Wash cells 3x for 5min with PBS.
Permeabilize cells in PBS containing 0.1% Triton for 5min at RT.
Block non-specific binding with Duolink PLA Blocking Solution (Sigma) for 30 min at RT.
Incubate cells with primary mouse anti-TLR9 antibody (antibodies-online, ABIN5542492, lot 160127) diluted 1:200 in staining solution ON at 4°C.
Wash cells 3x for 5min with PBS.
Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-Mouse IgG antibody (Jackson ImmunoResearch, 615-545-214) diluted 1:400 in staining solution for 1h at RT.
Wash cells 2x for 5min with PBS.
Mount coverslips on glass slides in ProLong Gold antifade reagent (Invitrogen, P36935,1890418) containing DAPI.
Image acquisition with an Olympus widefield microscope.

Experimental Notes

TLR9 staining using ABIN5542492 shows typical localization peri-nuclear localization.
生效 #103821 (Proximity Ligation Assay)

by

Klinik für Anästhesie, Intensivmedizin und Schmerztherapie, Universitätsklinikum der Ruhr Universität Bochum

No.

#103821

日期

2019.04.29

抗原

TLR9

Lot Number

160127

Method validated

Proximity Ligation Assay

Positive Control

U937 monocytic cells stimulated with ODN1826

Negative Control

Anti TLR9 antibody (ABIN5542492) alone

Anti pY antibody (ABIN361758) alone

Notes

Passed. PLA signal visible in stimulated cells. Negative control with only one primary antibody shows no signal.

Validation Images

Negative controls of U937 cells without either ABIN5542492 or ABIN361758 show no signal. In U937 cells incubated simulatnously with ABIN5542492 and ABIN361758 the PLA signal (green) is clearly visible. Counterstain with DAPI (blue).
Full Methods
Primary Antibody

ABIN5542492

Secondary Antibody

ABIN361758

Full Protocol

Grow U937 cells in RPMI (Gibco, 21875-034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep(Gibco, 15140-122), at 37°C and 5% CO₂ in 500µL in 12-well plates.
Incubate the cells with 1µM ODN1826 for 4h.
Use cytospin to spin cells onto slide.
Fix cells on coverslide in 4% PFA for 20min at RT.
Wash cells 3x for 5min with PBS.
Permeabilize cells in PBS containing 0.1% Triton for 5min at RT.
Block non-specific binding with Duolink PLA Blocking Solution (Sigma-Aldrich) for 30min at RT.
Incubate cells with primary mouse anti-TLR9 antibody (antibodies-online, ABIN5542492, lot 160127) diluted 1:200 and primary rabbit anti-pY antibody (antibodies-online, ABIN361758, lot 1508290) diluted 1:100 ON at 4°C.
Wash cells 3x for 5min with PBS.
Incubate cells with Duolink In Situ PLA Probe anti-Rabbit MINUS (Sigma-Aldrich, DUO92005, lot SLBZ4516) and Duolink In Situ PLA Probe anti-Mouse mouse PLUS (Sigma-Aldrich, DUO92001, lot SLBZ8369) proximity probes according to manufacturers recommendations.
Wash cells 2x for 5min with PBS.
Perform Ligation step according to the Duolink PLA kit (Sigma).
Perform Amplification step according to the Duolink In Situ PlA Detection Kit Green (Sigma-Aldrich, DUO92014).
Mount coverslips on glass slides in ProLong Gold antifade reagent (Invitrogen, P36935, lot 1890418) containing DAPI.
Image acquisition with an Olympus widefield microscope.

Experimental Notes

We used double the amount the Phi29 polymerase for the amplifaction step than recommended.
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

TLR9 (Toll-Like Receptor 9 (TLR9))

别名

TLR9 (TLR9 产品)

别名

CD289 antibody, toll like receptor 9 antibody, toll-like receptor 9 antibody, TLR9 antibody, Tlr9 antibody

背景

Description: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Aliases: CD289

分子量

115.8kDa

基因ID

54106

HGNC

54106

UniProt

Q9NR96

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades