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VEGFA 抗体 (AA 207-371)

Name: VEGFA 抗体 (AA 207-371)
Brand: antibodies-online
SKU: ABIN5542402
Availability: OutOfStock

VEGFA 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6G5A10 unconjugated

Western blot analysis using VEGFA mAb against human VEGFA (AA: 207-371) recombinant protein. (VEGFA 抗体 (AA 207-371))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (VEGFA 抗体 (AA 207-371))

ELISA

Western blot analysis using VEGFA mAb against HEK293 (1) and VEGFA (AA: 207-371)-hIgGFc transfected HEK293 (2) cell lysate. (VEGFA 抗体 (AA 207-371))

Western blot analysis using VEGFA mouse mAb against HUVEC (1), HEK293 (2), Jurkat (3), and Hela (4) cell lysate. (VEGFA 抗体 (AA 207-371))

Flow cytometric analysis of Hela cells using VEGFA mouse mAb (green) and negative control (red). (VEGFA 抗体 (AA 207-371))

FACS

5 images

产品编号 ABIN5542402

发货至: 中国

Datasheet as PDF

抗原 See all VEGFA 抗体

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
抗原表位
19

15

15

15

15

15

10

7

7

6

6

6

6

5

5

5

4

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 207-371
适用
159

129

101

22

18

18

17

16

10

5

5

4

3

2

2

2

2

1

1
人
宿主
210

53

12

1
小鼠
Compatible Secondaries
克隆类型
224

51

1
单克隆
标记
115

41

26

12

5

5

5

5

5

5

5

5

5

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This VEGFA antibody is un-conjugated
应用范围
198

91

65

65

52

29

24

21

20

16

15

12

10

10

8

4

3

2

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS)

原理

VEGFA Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human VEGFA (AA: 207-371) expressed in E. Coli.

克隆位点

6G5A10

亚型

IgG1

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应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

别名

VEGFA (VEGFA 产品)

别名

MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody

背景

Description: This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Aliases: VPF, VEGF, MVCD1

分子量

27kDa

基因ID

7422

HGNC

7422

UniProt

P15692

途径

RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling