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PDPK1 抗体 (AA 457-556)

Name: PDPK1 抗体 (AA 457-556)
Brand: antibodies-online
SKU: ABIN5542259
Availability: OutOfStock

PDPK1 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4G11 unconjugated

Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (PDPK1 抗体 (AA 457-556))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PDPK1 抗体 (AA 457-556))

ELISA

Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate. (PDPK1 抗体 (AA 457-556))

Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate. (PDPK1 抗体 (AA 457-556))

Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red). (PDPK1 抗体 (AA 457-556))

FACS

Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). (PDPK1 抗体 (AA 457-556))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining. (PDPK1 抗体 (AA 457-556))

IHC

7 images

产品编号 ABIN5542259

发货至: 中国

Datasheet as PDF

抗原 See all PDPK1 抗体

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
抗原表位
24

22

16

15

15

15

8

7

6

6

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 457-556
适用
174

120

83

10

6

6

6

5

5

3

3

2

1

1
人
宿主
173

17

1
小鼠
Compatible Secondaries
克隆类型
167

24
单克隆
标记
82

10

9

7

5

5

5

5

5

5

5

5

5

5

5

4

4

4

4

4

4

2

2
This PDPK1 antibody is un-conjugated
应用范围
141

65

53

53

27

27

12

9

8

7

6

4

3

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

原理

PDPK1 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

克隆位点

7E4G11

亚型

IgG1

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anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

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PDPK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Internal Region) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pTyr373), (pTyr376) antibody
PDPK1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

别名

PDPK1 (PDPK1 产品)

别名

pdpk1 antibody, MGC82080 antibody, PDK1 antibody, PDPK2 antibody, PRO0461 antibody, Pdk1 antibody, zgc:153787 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antibody, ATPDK1 antibody, AtPDK1 antibody, T32M21.110 antibody, T32M21_110 antibody, zgc:77318 antibody, 3-phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1 L homeolog antibody, 3-phosphoinositide dependent protein kinase-1 antibody, 3-phosphoinositide dependent protein kinase 1a antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1b antibody, PDPK1 antibody, pdpk1.L antibody, pdpk1 antibody, Pdpk1 antibody, pdpk1a antibody, LOC100380750 antibody, pdk-1 antibody, PDK1 antibody, pdpk1b antibody

背景

Description: Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.
Aliases: PDK1, PDPK2, PDPK2P, PRO0461

分子量

63.2kDa

基因ID

5170

HGNC

5170

UniProt

O15530

途径

PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals