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beta-Galactosidase Tag 抗体 (HRP)

WB, EIA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal HRP
产品编号 ABIN5541543
发货至: 中国
  • 抗原 See all beta-Galactosidase Tag products
    beta-Galactosidase Tag
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This beta-Galactosidase Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody detects Beta-galactosidase [ E. coli ]. Cross reactivity against Beta-galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, and anti-rabbit serum as well as purified and partially purified Beta-galactosidase [E.coli].
    纯化方法
    Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
    免疫原
    Beta-galactosidase isolated from E.coli
  • 应用备注
    Suitable for Immunoblotting (Western or dot blot), ELISA, Immunofluorescence microscopy, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Western blot : 1/5,000-1/10,000. A 1/5,000 dilution has been reported to be successful for staining by Immunoblot of Beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus). ELISA : 1/10,000. Immunohistochemistry : 1/1,500. The antibody recognizes both Frozen tissue sections, Paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis:A 1/1,500 dilution has been reported to detect Beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1/5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1/10,000 dilution was noted to show no background and to be suitable for double labeling experiments). A 1/5,000 dilution has been reported to be successful for staining brain sections from transgenic mice expressing nuclear Beta-galactosidase when assayed by Immunofluorescence microscopy .
    限制
    仅限研究用
  • 储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    beta-Galactosidase Tag
    Abstract
    beta-Galactosidase Tag 产品
    物质类
    Tag
    UniProt
    P00722
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