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FAS 抗体 (FITC)

Name: FAS 抗体 (FITC)
SKU: ABIN5541251
Availability: OutOfStock

FAS 适用: 人 FACS 宿主: 小鼠 Monoclonal UB2 FITC

(13 references)

产品编号 ABIN5541251

发货至: 中国

Datasheet as PDF

抗原 See all FAS 抗体

FAS (TNF Receptor Superfamily, Member 6 (FAS))
适用
216

61

59

19

5

4

4

2

1

1
人
宿主
156

96

3

1

1

1
小鼠
Compatible Secondaries
克隆类型
154

102

1
单克隆
标记
117

25

25

13

11

7

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This FAS antibody is conjugated to FITC
应用范围
159

119

71

57

37

33

26

26

26

19

16

13

8

5

5

4

2

2

2

2

2

1

1

1
Flow Cytometry (FACS)

特异性

This antibody recognizes the human Fas antigen specifically. Clone UB2 does not recognize the mouse Fas antigen.

纯化方法

Protein A agarose

免疫原

Recombinant human Fas

克隆位点

UB2

亚型

IgG1

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anti-TNF Receptor Superfamily, Member 6 (FAS) antibody
FAS 适用: 人 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 4F8H6 unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 87-109), (N-Term) antibody
FAS 适用: 大鼠, 小鼠 WB, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 22-172) antibody
FAS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 22-169) antibody
FAS 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 21-170) antibody
FAS 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 20-119) antibody
FAS 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2G3 unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 20-119) antibody
FAS 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 5B4 unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 1-335) antibody
FAS 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1B6 unconjugated

anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 26-96) antibody
FAS 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal FAS-3112 unconjugated

应用备注

Flow cytometry: 20 μL (ready for use). For details see protocol below.

实验流程

Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5 x 10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 20 μ L of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 μ L of FITC labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive control for Flow cytometry transfectant)

限制

仅限研究用
状态

Liquid

缓冲液

0.09 % NaN 3 Stabilizers: 1 % BSA

储存条件

4 °C

储存方法

Store at 2-8 °C. Shelf life: one year from despatch.

有效期

12 months
Mezosi, Wang, Utsugi, Bajnok, Bretz, Gauger, Thompson, Baker: "Induction and regulation of Fas-mediated apoptosis in human thyroid epithelial cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 3, pp. 804-11, (2005) (PubMed).

Hauptschein, Sloan, Torella, Moezzifard, Giel-Moloney, Zehetmeier, Unger, Ilag, Jay: "Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptosis." in: Cancer research, Vol. 65, Issue 5, pp. 1887-96, (2005) (PubMed).

Vega, Jazirehi, Huerta-Yepez, Bonavida et al.: "Rituximab-induced inhibition of YY1 and Bcl-xL expression in Ramos non-Hodgkin's lymphoma cell line via inhibition of NF-kappa B activity: role of YY1 and Bcl-xL in Fas resistance and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 4, pp. 2174-83, (2005) (PubMed).

Suhara, Fukuo, Yasuda, Tsubakimoto, Takemura, Kawamoto, Yokoi, Mogi, Kaimoto, Ogihara: "Homocysteine enhances endothelial apoptosis via upregulation of Fas-mediated pathways." in: Hypertension, Vol. 43, Issue 6, pp. 1208-13, (2004) (PubMed).

Suhara, Kim, Kirshenbaum, Walsh: "Suppression of Akt signaling induces Fas ligand expression: involvement of caspase and Jun kinase activation in Akt-mediated Fas ligand regulation." in: Molecular and cellular biology, Vol. 22, Issue 2, pp. 680-91, (2001) (PubMed).

Sata, Suhara, Walsh: "Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 2, pp. 309-16, (2000) (PubMed).

Shinohara, Yagita, Ikawa, Oyaizu: "Fas drives cell cycle progression in glioma cells via extracellular signal-regulated kinase activation." in: Cancer research, Vol. 60, Issue 6, pp. 1766-72, (2000) (PubMed).

Kuwano, Miyazaki, Hagimoto, Kawasaki, Fujita, Kunitake, Kaneko, Hara: "The involvement of Fas-Fas ligand pathway in fibrosing lung diseases." in: American journal of respiratory cell and molecular biology, Vol. 20, Issue 1, pp. 53-60, (1999) (PubMed).

Hiromatsu, Hoshino, Yagita, Koga, Sakisaka, Honda, Yang, Kayagaki, Okumura, Nonaka: "Functional Fas ligand expression in thyrocytes from patients with Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 84, Issue 8, pp. 2896-902, (1999) (PubMed).

Dai, Price, Brunner, Krantz: "Fas ligand is present in human erythroid colony-forming cells and interacts with Fas induced by interferon gamma to produce erythroid cell apoptosis." in: Blood, Vol. 91, Issue 4, pp. 1235-42, (1998) (PubMed).

Boirivant, Pica, DeMaria, Testi, Pallone, Strober: "Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis." in: The Journal of clinical investigation, Vol. 98, Issue 11, pp. 2616-22, (1997) (PubMed).

Ando, Hiroishi, Kaneko, Moriyama, Muto, Kayagaki, Yagita, Okumura, Imawari: "Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5283-91, (1997) (PubMed).

Hata, Matsuzaki, Takeya, Yoshida, Sonoki, Nagasaki, Kuribayashi, Kawano, Takatsuki: "Expression of Fas/Apo-1 (CD95) and apoptosis in tumor cells from patients with plasma cell disorders." in: Blood, Vol. 86, Issue 5, pp. 1939-45, (1995) (PubMed).
抗原

FAS (TNF Receptor Superfamily, Member 6 (FAS))

别名

cd95,fas (FAS 产品)

背景

It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, includin g tumor necrosis factor receptor and nerve growth factor receptor.

UniProt

P25445

途径

p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity