Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunocytochemistry (ICC)
特异性
The antibody recognizes Carboxypeptidase Y from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Species: Bakers Yeast.
纯化方法
Solid Phase Affinity Chromatography
免疫原
Carboxypeptidase Y isolated and purified from Baker's yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/100,000.
限制
仅限研究用
缓冲液
PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
储存液
Without preservative
储存条件
4 °C,-20 °C
储存方法
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Shelf life: one year from despatch.