Isocitrate Dehydrogenase 抗体
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- 抗原 See all Isocitrate Dehydrogenase (IDH) 抗体
- Isocitrate Dehydrogenase (IDH)
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适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Isocitrate Dehydrogenase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunocytochemistry (ICC)
- 特异性
- Isocitrate Dehydrogenase isolated and purified from Porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Species: Pig.
- 纯化方法
- Solid Phase Affinity Chromatography
- 免疫原
- Isocitrate Dehydrogenase isolated and purified from Porcine heart. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product IDH Primary Antibody
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anti-Isocitrate Dehydrogenase (IDH) antibody
IDH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase (IDH) antibodyIDH 适用: Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Zea mays WB 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase (IDH) (Middle Region) antibodyIDH 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Immunoprecipiation. Can be Used: • In precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). • To prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/30,000.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- 有效期
- 12 months
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- 抗原
- Isocitrate Dehydrogenase (IDH)
- 别名
- isocitrate dehydrogenase,idh (IDH 产品)
- 别名
- CG7176 antibody, CT22171 antibody, Dmel\\CG7176 antibody, IDH antibody, IDH-NADP antibody, Idh-NADP antibody, idh antibody, isocitrate dehydrogenase antibody, l(3)L3852 antibody, F20D21.16 antibody, F20D21_16 antibody, SMU.672 antibody, icd antibody, Isocitrate dehydrogenase antibody, isocitrate dehydrogenase antibody, isocitrate dehydrogenase, NADP-dependent antibody, Monomeric isocitrate dehydrogenase antibody, ankyrin repeat domain 26-like antibody, Idh antibody, ICDH antibody, idh antibody, icd antibody, SSO_RS10660 antibody, JK_RS08640 antibody, LOC618625 antibody
- 背景
- Isocitrate dehydrogenase is an enzyme involved in the citric acid cycle. It is 416 amino acids long with a molecular weight of approximately 45 kDa. Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction. Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.
- UniProt
- P20304
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