STAT5A 抗体 (AA 681-692)
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- 抗原 See all STAT5A 抗体
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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抗原表位
- AA 681-692
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This STAT5A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- 原理
- STAT5A (aa681-692)
- 序列
- KYYTPVLAKA VD
- 特异性
- This antibody is not expected to cross-react with STAT5B.
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product STAT5A Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 100 kDa band observed in lysates of cell line K562 (calculated MW of 90.6 kDa according to NP_003143.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
- 别名
- STAT5A (STAT5A 产品)
- 背景
- STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5
- 基因ID
- 6776, 20850, 24918
- NCBI登录号
- NP_003143
- 途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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