STAT5A 抗体 (AA 681-692)
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- 抗原 See all STAT5A 抗体
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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抗原表位
- AA 681-692
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This STAT5A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- 原理
- STAT5A (aa681-692)
- 序列
- KYYTPVLAKA VD
- 特异性
- This antibody is not expected to cross-react with STAT5B.
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product STAT5A Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 100 kDa band observed in lysates of cell line K562 (calculated MW of 90.6 kDa according to NP_003143.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature
Peptide ELISA: antibody detection limit dilution 1:8000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
- 别名
- STAT5A (STAT5A 产品)
- 别名
- MGF antibody, STAT5 antibody, AA959963 antibody, Stat5 antibody, STAT5A/MGF antibody, STATA5 antibody, STAT5A antibody, stat5 antibody, stat5b antibody, signal transducer and activator of transcription 5A antibody, signal transducer and activator of transcription 5a antibody, STAT5A antibody, Stat5a antibody, stat5a antibody, LOC100348518 antibody, LOC100716811 antibody
- 背景
- STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5
- 基因ID
- 6776, 20850, 24918
- NCBI登录号
- NP_003143
- 途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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