HSPD1 抗体 (AA 333-344)
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- 抗原 See all HSPD1 抗体
- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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抗原表位
- AA 333-344
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适用
- 人, 大鼠, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This HSPD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
- 原理
- HSP60 / HSPD1 (aa333-344)
- 序列
- NLEDVQPHDL GK
- 特异性
- Reported variants represent identical protein: NP_955472.1, NP_002147.2.
- 交叉反应
- Cow, 犬, 人, Pig
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product HSPD1 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 60 kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1 kDa according to Human NP_002147.2 and 60.9 kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentrat
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
- 别名
- HSPD1 (HSPD1 产品)
- 背景
- HSPD1, heat shock 60 kDa protein 1 (chaperonin), CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, chaperonin 60, heat shock protein 65, mitochondrial matrix prote
- 基因ID
- 3329
- NCBI登录号
- NP_002147
- 途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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