EGF 抗体 (Internal Region)
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- 抗原 See all EGF 抗体
- EGF (Epidermal Growth Factor (EGF))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This EGF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- Pro-EGF
- 序列
- SRQERVCNIE KNVS
- 特异性
- This antibody is expected to recognize all reported isoforms (NP_001954.2, NP_001171601.1, NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
- 交叉反应
- 犬, 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product EGF Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 5 μg/mL.
Western Blot: Approx 160 kDa double band observed in Human Prostate lysates (calculated MW of 134 kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3 μg/mL. Primary incubation was 1
Peptide ELISA: antibody detection limit dilution 1:128000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- 别名
- EGF (EGF 产品)
- 别名
- HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
- 背景
- EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor
- 基因ID
- 1950
- NCBI登录号
- NP_001954, NP_001171601, NP_001171602
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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