This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the C-terminal region of human UBE2H.
UBE2H
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 594
应用备注
For WB starting dilution is: 1:1000
限制
仅限研究用
状态
Liquid
浓度
0.42 mg/mL
缓冲液
Supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100 % identical to the mouse homolog and 98 % identical to the frog and zebrafish homologs, which indicates that this enzyme is highly conserved in eukaryotes.